EMPIAR-11441
FMC63 scFv in complex with soluble CD19 [multiple data sets in TIFF format]
Publication:

CD19 CAR antigen engagement mechanisms and affinity tuning

He C, Mansilla-Soto J, Khanra N, Hamieh M, Bustos V, Paquette AJ, Garcia Angus A, Shore DM, Rice WJ, Khelashvili G, Sadelain M, Meyerson JR

Science immunology 8 (2023)

PMID: 36867678

Related EMDB entry:
Deposited:
2023-01-16
Released:
2023-03-17
Last modified:
2023-03-17
Imageset size:
1.45 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. FMC63-CD19 dataset1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
489
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.548, 0.548)
Details:
Arctica operated at 200 kV with a GatanK3 imaging system collected at 36,000X nominal magnification. The calibrated pixel size of 0.5480 Å was used for processing.
Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 21.17 e-/Å2/s with a total exposure of 2.40 seconds, for an accumulated dose of 50.82 e-/Å2. Intermediate frames were recorded every 0.05 seconds for a total of 48 frames per micrograph.
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2. FMC63-CD19 dataset2
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1910
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.548, 0.548)
Details:
Arctica operated at 200 kV with a GatanK3 imaging system collected at 36,000X nominal magnification. The calibrated pixel size of 0.5480 Å was used for processing.
Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 21.00 e-/Å2/s with a total exposure of 2.40 seconds, for an accumulated dose of 50.40 e-/Å2. Intermediate frames were recorded every 0.05 seconds for a total of 48 frames per micrograph.
Files:
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