Structure of RecT protein from Listeria innoccua phage A118 in complex with 83-mer single stranded DNA [1619 multi-frame micrographs composed of 45 frames each in TIFF format]
Publication:
Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing
Data collected on 11/23/20-11/24/20 Krios, K3, counted mode, EFTEM, Cs corrector, super-resolution User/Group:Brian Caldwell/Chuck Bell
Image information: Total # of images = 1633 selected, 22 grid squares Total data size = 0.933 TB Rate of collection = 69/hour Mode of collection = Accurate hole centering Gain normalized = NO File format = LZW tiff Super-resolution = YES Spherical aberration = 0.01 mm Images per hole = 1 Exposure time = 2.83 s Fractions = 45
Microscope parameters: Pixel size = 0.899 A Magnification = 81,000 Stage tilt = 0 deg Dose rate = 18.43 e/px/s Dose rate = 22.80 e/A^2/s Total dose = 65 e/A^2 [desired dose rate] Defocus range = -3.5, -3, -2.5, -2, -1.5, -1 C2 aperture = 50 um Objective aperture= 100 um Illuminated area = 1.5 um Energy Filter = YES Slit width = 20 eV
Grid type = Quantifoil (gold coated) R1.2/1.3 300 Mesh hole size, mesh, etc. Position 1 of the large dewar Bell Conical: Position 1 of box F2 (Purple) Prepared on [date] by Brian Caldwell
Additional notes: Copied gain reference file over into the data folder. Gain reference image located at: /data/gain.mrc Saved EPU settings file.
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Archives and downloads the selected files into an uncompressed zip file.
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