EMPIAR-11321 - LRRC8A-BRIL(T48D):C Heteromer in GDN [multiple data sets in TIFF and MRCS formats]

EMPIAR-11321

LRRC8A-BRIL(T48D):C Heteromer in GDN [multiple data sets in TIFF and MRCS formats]

Publication:

Structural basis for assembly and lipid-mediated gating of LRRC8A:C volume-regulated anion channels

Kern DM, Bleier J, Mukherjee S, Hill JM, Kossiakoff AA, Isacoff EY, Brohawn SG

bioRxiv (2022)

PMID: 30775971

DOI: 10.1101/2022.07.31.502239

Related EMDB entry:

EMD-28901 EMD-28904 EMD-28895 EMD-28905 EMD-28897 EMD-28894 EMD-28903 EMD-28898

Deposited:

2022-11-22

Released:

2023-03-14

Last modified:

2023-03-14

Imageset size:

2.06 TB

Imageset DOI:

10.6019/EMPIAR-11321

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - multiframe - processed

Image Sets

1. Unaligned tif movies for LRRC8A-BRIL(T48D):C in GDN

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6939

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.048, 1.048)

Details:

Gain reference in mrc form is included in the folder with the movies. In RELION, the gain must be flipped upside down. To match our motion correction settings, use 0.9957 e/Å2 dose per frame and do not bin during motion correction.

Files:

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2. Polished particles stacks for LRRC8A-BRIL(T48D)/C in GDN

Category:

picked particles - multiframe - processed

Image format:

MRCS

No. of images or tilt series:

5988

Image size:

(416, 416)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.048, 1.048)

Details:

Contains polished particles (145,915) as 5988 mrcs files. The selections of particles for each conformation (1 and 2) are in the EMPIARparticles folder. They are in RELION star format and can be imported into cryoSPARC as well for processing.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

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