EMPIAR-11321
LRRC8A-BRIL(T48D):C Heteromer in GDN [multiple data sets in TIFF and MRCS formats]
Publication:

Structural basis for assembly and lipid-mediated gating of LRRC8A:C volume-regulated anion channels

Kern DM, Bleier J, Mukherjee S, Hill JM, Kossiakoff AA, Isacoff EY, Brohawn SG

bioRxiv (2022)

PMID: 30775971

Deposited:
2022-11-22
Released:
2023-03-14
Last modified:
2023-03-14
Imageset size:
2.06 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
  • picked particles - multiframe - processed
1. Unaligned tif movies for LRRC8A-BRIL(T48D):C in GDN
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
6939
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.048, 1.048)
Details:
Gain reference in mrc form is included in the folder with the movies. In RELION, the gain must be flipped upside down. To match our motion correction settings, use 0.9957 e/Å2 dose per frame and do not bin during motion correction.
Files:
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2. Polished particles stacks for LRRC8A-BRIL(T48D)/C in GDN
Category:
picked particles - multiframe - processed
Image format:
MRCS
No. of images or tilt series:
5988
Image size:
(416, 416)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.048, 1.048)
Details:
Contains polished particles (145,915) as 5988 mrcs files. The selections of particles for each conformation (1 and 2) are in the EMPIARparticles folder. They are in RELION star format and can be imported into cryoSPARC as well for processing.
Files:
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Files:
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Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)