EMPIAR-11305 - Reconstituted USP1, UAF1, FANCI, and mono-ubiquitinated FANCD2 [4593 multi-frame micrographs composed of 59 frames each in TIFF format]

EMPIAR-11305

Reconstituted USP1, UAF1, FANCI, and mono-ubiquitinated FANCD2 [4593 multi-frame micrographs composed of 59 frames each in TIFF format]

Publication:

Structural basis of FANCD2 deubiquitination by USP1-UAF1

Rennie ML, Arkinson C, Chaugule VK, Toth R, Walden H

Nature structural & molecular biology 28 (2021) 356-364

PMID: 33795880

DOI: 10.1038/s41594-021-00576-8

Related EMDB entry:

EMD-11934

Deposited:

2022-11-14

Released:

2022-12-05

Last modified:

2022-12-05

Imageset size:

1.60 TB

Imageset DOI:

10.6019/EMPIAR-11305

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of reconstituted USP1, UAF1, FANCI, monoubiquitinated FANCD2, and DNA

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4593

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.015, 1.015)

Details:

Multi-frame raw micrographs without gain correction in LZW compressed TIFF were generated from gain-corrected MRCs using relion_convert_to_tiff. Two batches of micrographs are uploaded (data/batch*/*.tif), each with a separate gain reference. Imaging was performed at 120,000x magnification on JEOL CryoARM 300 equipped with a DE64 detector at SCMI on 2020-08-17.
Sample contained 8.8 μM of each of USP1(C90S), UAF1, FANCI, FANCD2 monoubiquitinated on K561 and dsDNA (61 base pairs).

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