EMPIAR-11305
Reconstituted USP1, UAF1, FANCI, and mono-ubiquitinated FANCD2 [4593 multi-frame micrographs composed of 59 frames each in TIFF format]
Publication:

Structural basis of FANCD2 deubiquitination by USP1-UAF1

Rennie ML, Arkinson C, Chaugule VK, Toth R, Walden H

Nature structural & molecular biology 28 (2021) 356-364

PMID: 33795880

Related EMDB entry:
Deposited:
2022-11-14
Released:
2022-12-05
Last modified:
2022-12-05
Imageset size:
1.60 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of reconstituted USP1, UAF1, FANCI, monoubiquitinated FANCD2, and DNA
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
4593
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.015, 1.015)
Details:
Multi-frame raw micrographs without gain correction in LZW compressed TIFF were generated from gain-corrected MRCs using relion_convert_to_tiff. Two batches of micrographs are uploaded (data/batch*/*.tif), each with a separate gain reference. Imaging was performed at 120,000x magnification on JEOL CryoARM 300 equipped with a DE64 detector at SCMI on 2020-08-17.
Sample contained 8.8 μM of each of USP1(C90S), UAF1, FANCI, FANCD2 monoubiquitinated on K561 and dsDNA (61 base pairs).
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