EMPIAR-11286
Cryo-EM Structure of Formate Dehydrogenase 1 from Methylorubrum extorquens AM1 [7650 multi-frame micrographs composed of 40 frames each in TIFF format]
Publication:

Multiple electron transfer pathways of tungsten-containing formate dehydrogenase in direct electron transfer-type bioelectrocatalysis

Yoshikawa T, Makino F, Miyata T, Suzuki Y, Tanaka H, Namba K, Kano K, Sowa K, Kitazumi Y, Shirai O

Chemical communications (Cambridge, England) 58 (2022) 6478-6481

PMID: 35535582

Related EMDB entry:
Deposited:
2022-10-25
Released:
2022-11-28
Last modified:
2022-11-28
Imageset size:
1.44 TB
Imageset DOI:
Experimental metadata:
Download xml json
Deposited via PDBj
Contains:
  • micrographs - multiframe
1. Raw movies
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
7650
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.87, 0.87)
Details:
300 kV
Cs 2.7 mm
2.0 e/Å2/frame

Note that the pixel size in the image header (0.767 Å/px) is wrong. 0.87 Å/px is the calibrated value.

Please use CountCDSRef_FoDH_5mg_Au_45-2_000.mrc as a gain reference without rotation or flipping.

The last three digits (000 to 074) in the movie file names represent the beam shift directions: 5 x 5 (holes) x 3 (images per hole). The preceding digits (e.g. 48-22) are the stage positions.
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