EMPIAR-11264 - Cryo-electron tomography of microtubules assembled from purified porcine brain tubulin in the presence of GMPCPP [8 tilt series in MRC format]

EMPIAR-11264

Cryo-electron tomography of microtubules assembled from purified porcine brain tubulin in the presence of GMPCPP [8 tilt series in MRC format]

Publication:

Changes in seam number and location induce holes within microtubules assembled from porcine brain tubulin and in Xenopus egg cytoplasmic extracts

Guyomar C, Bousquet C, Ku S, Heumann J, Guilloux G, Gaillard N, Heichette C, Duchesne L, Steinmetz MO, Gibeaux R, Chrétien D

bioRxiv (2021)

DOI: 10.1101/2021.07.14.452321

Related EMDB entry:

EMD-15740

Deposited:

2022-08-29

Released:

2022-11-14

Last modified:

2022-11-14

Imageset size:

76.11 GB

Imageset DOI:

10.6019/EMPIAR-11264

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Single- and dual-axis tilt series used to perform segmented sub-tomogram averaging of microtubules assembled in the presence of GMPCPP and decorated with kinesin motor domains

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(None, None)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(None, None)

Details:

Single- and dual-axis tilt series. The number of images in the tilt series is variable.
GMPCPP_S1_tomo1, GMPCPP_S1_tomo2, GMPCPP_S1_tomo3 and GMPCPP_S1_tomo4 are dual-axis tilt series, with a pixel spacing of 7.9 Å (image size 2048, 2048).
GMPCPP_S2_tomo1 and GMPCPP_S2_tomo2 are single-axis tilt series, with a pixel size of 3.7 Å (image size 4096,4096).
GMPCPP_tomoFig5 and GMPCPP_tomoFig6 are single-axis tilt series, with a pixel size of 2.1 Å (image size 4096,4096).

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Talks and Tutorials

EMPIAR Quick tour

Statistics

Publications

Re-use case study

EMPIAR in the news

Claim entries to your ORCiD

COVID-19 Data Portal

EMDB

PDBe

BioImage Archive

EMNavigator@PDBj

PDBj

RCSB@PDBj

EMDataResoruce@RCSB,EMDB,NCMI

Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

Protein Data Bank Japan News

[2024-11-20]

224 new PDB entries have been released on 2024-11-20.

[2024-11-01]

A new portal integrating genome variants and protein structures has released

[2024-10-30]

[wwPDB] Deprecation of FTP File Download Protocol on November 1, 2024

[2024-10-25]

PDBj luncheon seminar at APBJC 2024

[2024-10-17]

[wwPDB] The Nobel Prize in Chemistry 2024