EMPIAR-11264
Cryo-electron tomography of microtubules assembled from purified porcine brain tubulin in the presence of GMPCPP [8 tilt series in MRC format]
Publication:

Changes in seam number and location induce holes within microtubules assembled from porcine brain tubulin and in Xenopus egg cytoplasmic extracts

Guyomar C, Bousquet C, Ku S, Heumann J, Guilloux G, Gaillard N, Heichette C, Duchesne L, Steinmetz MO, Gibeaux R, Chrétien D

bioRxiv (2021)

Related EMDB entry:
Deposited:
2022-08-29
Released:
2022-11-14
Last modified:
2022-11-14
Imageset size:
76.11 GB
Imageset DOI:
Experimental metadata:
Contains:
  • tilt series
1. Single- and dual-axis tilt series used to perform segmented sub-tomogram averaging of microtubules assembled in the presence of GMPCPP and decorated with kinesin motor domains
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
8
Image size:
(None, None)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Single- and dual-axis tilt series. The number of images in the tilt series is variable.
GMPCPP_S1_tomo1, GMPCPP_S1_tomo2, GMPCPP_S1_tomo3 and GMPCPP_S1_tomo4 are dual-axis tilt series, with a pixel spacing of 7.9 Å (image size 2048, 2048).
GMPCPP_S2_tomo1 and GMPCPP_S2_tomo2 are single-axis tilt series, with a pixel size of 3.7 Å (image size 4096,4096).
GMPCPP_tomoFig5 and GMPCPP_tomoFig6 are single-axis tilt series, with a pixel size of 2.1 Å (image size 4096,4096).
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Citations
Liu T, Shilliday F, Cook AD, Zeeshan M, Brady D, Tewari R, Sutherland CJ, Roberts AJ, Moores CA. (2022)
Jespersen N, Ehrenbolger K, Winiger RR, Svedberg D, Vossbrinck CR, Barandun J. (2022)
Sheng Y, Harrison PJ, Vogirala V, Yang Z, Strain-Damerell C, Frosio T, Himes BA, Siebert CA, Zhang P, Clare DK. (2022)
See all citations
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