EMPIAR-11121 - Single particle cryo-EM dataset of the Vairimorpha necatrix 26S proteasome from sporoplasms [2658 multi-frame micrographs composed of 2700 frames each in EER format]

EMPIAR-11121

Single particle cryo-EM dataset of the Vairimorpha necatrix 26S proteasome from sporoplasms [2658 multi-frame micrographs composed of 2700 frames each in EER format]

Publication:

Structure of the reduced microsporidian proteasome bound by PI31-like peptides in dormant spores

Jespersen N, Ehrenbolger K, Winiger RR, Svedberg D, Vossbrinck CR, Barandun J

Nature communications 13 (2022)

PMID: 36379934

DOI: 10.1038/s41467-022-34691-x

Related EMDB entry:

EMD-15366

Deposited:

2022-07-18

Released:

2022-12-13

Last modified:

2022-12-13

Imageset size:

3.72 TB

Imageset DOI:

10.6019/EMPIAR-11121

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame images of the Vairimorpha necatrix proteasome from sporoplasms

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

2658

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(1.042, 1.042)

Details:

EER-format movies with 2700 frames were collected at a total dose 40.0 electrons per squared angstrom over an exposure time of 8.78 seconds. Defocus range of 1.2–3.0 μm, pixel size of 1.495 Å. Collected on 200kV Glacios with at Falcon 4i detector.

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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