EMPIAR-11121
Single particle cryo-EM dataset of the Vairimorpha necatrix 26S proteasome from sporoplasms [2658 multi-frame micrographs composed of 2700 frames each in EER format]
Publication:

Structure of the reduced microsporidian proteasome bound by PI31-like peptides in dormant spores

Jespersen N, Ehrenbolger K, Winiger RR, Svedberg D, Vossbrinck CR, Barandun J

Nature communications 13 (2022)

PMID: 36379934

Related EMDB entry:
Deposited:
2022-07-18
Released:
2022-12-13
Last modified:
2022-12-13
Imageset size:
3.72 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Unaligned multi-frame images of the Vairimorpha necatrix proteasome from sporoplasms
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
2658
Image size:
(4096, 4096)
Pixel type:
BIT
Pixel spacing:
(1.042, 1.042)
Details:
EER-format movies with 2700 frames were collected at a total dose 40.0 electrons per squared angstrom over an exposure time of 8.78 seconds. Defocus range of 1.2–3.0 μm, pixel size of 1.495 Å. Collected on 200kV Glacios with at Falcon 4i detector.
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