EMPIAR-11070 - Tilt series of SARS-CoV-2 virions, Wuhan/Alpha/Beta/Delta variants [multiple data sets in MRC format]

EMPIAR-11070

Tilt series of SARS-CoV-2 virions, Wuhan/Alpha/Beta/Delta variants [multiple data sets in MRC format]

Publication:

Electron cryotomography of SARS-CoV-2 virions reveals cylinder-shaped particles with a double layer RNP assembly

Calder LJ, Calcraft T, Hussain S, Harvey R, Rosenthal PB

Communications Biology 5 (2022)

DOI: 10.1038/s42003-022-04183-1

Related EMDB entry:

EMD-15183 EMD-15185 EMD-15181 EMD-15182

Deposited:

2022-06-15

Released:

2022-11-11

Last modified:

2022-11-11

Imageset size:

120.53 GB

Imageset DOI:

10.6019/EMPIAR-11070

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
tilt series

Image Sets

1. Unaligned tilt movies of SARS-CoV-2 virions (Wuhan strain)

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

533

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(2.22, 2.22)

Details:

Movies have 4 frames each, corresponding to 2.38e-/A^2 per tilt. Tilt series collected +/-60deg in dose symmetric sequence, grouping 2 (0,+3,-3,-6,+6,+9,etc...). Tilt axis rotation angle is 177deg.

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Tilt series of SARS-CoV-2 virions (Alpha variant B.1.1.7)

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(2.58, 2.58)

Details:

2.5e-/A^2 dose per tilt. Tilt series collected +/-57deg in dose symmetric sequence, grouping 2 (0,+3,-3,-6,+6,+9,etc...). Tilt axis rotation angle is -2deg.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Tilt series of SARS-CoV-2 virions (Beta variant B.1.351)

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(2.58, 2.58)

Details:

2.5e-/A^2 dose per tilt. Tilt series collected +/-57deg in dose symmetric sequence, grouping 2 (0,+3,-3,-6,+6,+9,etc...). Tilt axis rotation angle is -2deg.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Tilt series of SARS-CoV-2 virions (Delta variant B.1.617)

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(2.58, 2.58)

Details:

2.0e-/A^2 dose per tilt. Tilt series collected +/-60deg in dose symmetric sequence, grouping 2 (0,+3,-3,-6,+6,+9,etc...). Tilt axis rotation angle is -2deg.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

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