EMPIAR-11070
Tilt series of SARS-CoV-2 virions, Wuhan/Alpha/Beta/Delta variants [multiple data sets in MRC format]
Publication:

Electron cryotomography of SARS-CoV-2 virions reveals cylinder-shaped particles with a double layer RNP assembly

Calder LJ, Calcraft T, Hussain S, Harvey R, Rosenthal PB

Communications Biology 5 (2022)

Related EMDB entry:
Deposited:
2022-06-15
Released:
2022-11-11
Last modified:
2022-11-11
Imageset size:
129.42 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • tilt series
1. Unaligned tilt movies of SARS-CoV-2 virions (Wuhan strain)
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
533
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(2.22, 2.22)
Details:
Movies have 4 frames each, corresponding to 2.38e-/A^2 per tilt. Tilt series collected +/-60deg in dose symmetric sequence, grouping 2 (0,+3,-3,-6,+6,+9,etc...). Tilt axis rotation angle is 177deg.
Files:
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2. Tilt series of SARS-CoV-2 virions (Alpha variant B.1.1.7)
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
2
Image size:
(4096, 4096)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(2.58, 2.58)
Details:
2.5e-/A^2 dose per tilt. Tilt series collected +/-57deg in dose symmetric sequence, grouping 2 (0,+3,-3,-6,+6,+9,etc...). Tilt axis rotation angle is -2deg.
Files:
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3. Tilt series of SARS-CoV-2 virions (Beta variant B.1.351)
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
2
Image size:
(4096, 4096)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(2.58, 2.58)
Details:
2.5e-/A^2 dose per tilt. Tilt series collected +/-57deg in dose symmetric sequence, grouping 2 (0,+3,-3,-6,+6,+9,etc...). Tilt axis rotation angle is -2deg.
Files:
Loading...
4. Tilt series of SARS-CoV-2 virions (Delta variant B.1.617)
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
2
Image size:
(4096, 4096)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(2.58, 2.58)
Details:
2.0e-/A^2 dose per tilt. Tilt series collected +/-60deg in dose symmetric sequence, grouping 2 (0,+3,-3,-6,+6,+9,etc...). Tilt axis rotation angle is -2deg.
Files:
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