EMPIAR-10860 - In situ cryo-electron tomography of E. coli APEC 2248 infected by Goslar [1073 tilt series in TIFF format]

EMPIAR-10860

In situ cryo-electron tomography of E. coli APEC 2248 infected by Goslar [1073 tilt series in TIFF format]

Publication:

Architecture and self-assembly of the jumbo bacteriophage nuclear shell

Laughlin TG, Deep A, Prichard AM, Seitz C, Gu Y, Enustun E, Suslov S, Khanna K, Birkholz EA, Armbruster E, McCammon JA, Amaro RE, Pogliano J, Corbett KD, Villa E

bioRxiv (2022)

DOI: 10.1101/2022.02.14.480162

Related EMDB entry:

EMD-25229 EMD-25360 EMD-25358 EMD-25359 EMD-25262

Deposited:

2021-11-07

Released:

2022-07-26

Last modified:

2022-07-26

Imageset size:

52.19 GB

Imageset DOI:

10.6019/EMPIAR-10860

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Unaligned tilt-movie frames as unormalized LZW-TIFF

Category:

tilt series

Image format:

TIFF

No. of images or tilt series:

1073

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(4.265, 4.265)

Details:

Gain reference supplied in data/frames as MRC and requires a 270 CW rotation prior to application to TIFs. Fluence per tilt-movie is 1.8 electrons per square Angstrom. SerialEM metadata for the 19 tilt-series is in data/mdocs, Warp/M metadata in data/xmls_and_tomostars, and positions of ribosomes in data/ribosome_positions as M formatted .star files.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

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