EMPIAR-10796 - A microtubule RELION-based pipeline for cryo-EM image processing [multiple data sets in TIFF format]

EMPIAR-10796

A microtubule RELION-based pipeline for cryo-EM image processing [multiple data sets in TIFF format]

Publication:

A microtubule RELION-based pipeline for cryo-EM image processing

Cook AD, Manka SW, Wang S, Moores CA, Atherton J

Journal of Structural Biology (2019)

PMID: 31610239

DOI: 10.1016/j.jsb.2019.10.004

Related EMDB entry:

EMD-10131

Deposited:

2019-07-24

Released:

2021-09-24

Last modified:

2022-02-04

Imageset size:

256.73 GB

Imageset DOI:

10.6019/EMPIAR-10796

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Movies of microtubules decorated with MKLP2 motor domain (ADPAlFx bound)

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

293

Image size:

(4880, 3600)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.53, 1.53)

Details:

22 frames over 1.5 seconds (frames tiff format, dark and gain reference frames provided for correction).
50 electrons per squared Angstrom total dose over the whole movie.
Pixel size 1.53 Angstrom/Pixel.
Camera and microscope: DE20 direct detector in linear mode on a Technai Polara at 300kV.

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2. Single-frames of microtubules decorated with MKLP2 motor domain (ADPAlFx bound)

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

6741

Image size:

(5120, 3840)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.53, 1.53)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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