EMPIAR-10796
A microtubule RELION-based pipeline for cryo-EM image processing [multiple data sets in TIFF format]
Publication:

A microtubule RELION-based pipeline for cryo-EM image processing

Cook AD, Manka SW, Wang S, Moores CA, Atherton J

Journal of Structural Biology (2019)

PMID: 31610239

Related EMDB entry:
Deposited:
2019-07-24
Released:
2021-09-24
Last modified:
2022-02-04
Imageset size:
256.73 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - single frame
1. Movies of microtubules decorated with MKLP2 motor domain (ADPAlFx bound)
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
293
Image size:
(4880, 3600)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(1.53, 1.53)
Details:
22 frames over 1.5 seconds (frames tiff format, dark and gain reference frames provided for correction).
50 electrons per squared Angstrom total dose over the whole movie.
Pixel size 1.53 Angstrom/Pixel.
Camera and microscope: DE20 direct detector in linear mode on a Technai Polara at 300kV.
Files:
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2. Single-frames of microtubules decorated with MKLP2 motor domain (ADPAlFx bound)
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
6741
Image size:
(5120, 3840)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(1.53, 1.53)
Details:
22 frames over 1.5 seconds (frames tiff format, dark and gain reference frames provided for correction).
50 electrons per squared Angstrom total dose over the whole movie.
Pixel size 1.53 Angstrom/Pixel.
Camera and microscope: DE20 direct detector in linear mode on a Technai Polara at 300kV.
Files:
Loading...
Files:
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