EMPIAR-10731 - Locating Macromolecular Assemblies in Cells by 2D Template Matching with cisTEM [multiple data sets in MRC format]

EMPIAR-10731

Locating Macromolecular Assemblies in Cells by 2D Template Matching with cisTEM [multiple data sets in MRC format]

Publication:

Locating macromolecular assemblies in cells by 2D template matching with cisTEM

Lucas BA, Himes BA, Xue L, Grant T, Mahamid J, Grigorieff N

eLife 10 (2021)

PMID: 34114559

DOI: 10.7554/elife.68946

Related EMDB entry:

EMD-11999

Deposited:

2021-06-12

Released:

2021-11-15

Last modified:

2021-11-15

Imageset size:

67.79 GB

Imageset DOI:

10.6019/EMPIAR-10731

Experimental metadata:

Download xml json

Contains:

reconstructed volumes
micrographs - single frame
tilt series

Image Sets

1. 50S templates of Mycoplasma pneumoniae used for 2D and 3D template matching

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

Image size:

(None, None)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(None, None)

Details:

3D density maps used as templates to detect 50S ribosomal subunits the 2D images and 3D tomograms of Mycoplasma pneumoniae cells by 2D and 3D template matching, respectively. The pixel size of the map used for 2D template matching is 1.27 Å. The map used for 3D template matching has a pixel size of 6.80 Å.

Files:

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2. Single-exposure micrographs of untilted Mycoplasma pneumoniae cells

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

Image size:

(1919, 1855)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.27, 1.27)

Details:

19 micrographs of Mycoplasma pneumoniae cells, using movies of 20 frames and a total exposures of 32 e-/Å^2. The movies were aligned using cisTEM, and exposure weighting was applied to calculate the frame average.

Files:

Archives and downloads the selected files into an uncompressed zip file.

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It is possible to download files by specifying the file list with rsync command, etc.

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3. Tomographic tilt series of Mycoplasma pneumoniae cells

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3708, 3708)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.7, 1.7)

Details:

19 aligned tomographic tilt series of Mycoplasma pneumoniae cells collected using SerialEM. Tilts ranged from -60 to 60 deg using 3 deg angular steps. The total exposure was 129 e-/Å^2. The tilt series were recorded after a pre-exposure of 32 e-/Å^2 to collect a 2D image of each cell at higher resolution.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Tomographic reconstructions of Mycoplasma pneumoniae cells

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

Image size:

(928, 928)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(6.8, 6.8)

Details:

19 tomographic reconstructions of Mycoplasma pneumoniae cells

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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