EMPIAR-10731
Locating Macromolecular Assemblies in Cells by 2D Template Matching with cisTEM [multiple data sets in MRC format]
Publication:

Locating macromolecular assemblies in cells by 2D template matching with cisTEM

Lucas BA, Himes BA, Xue L, Grant T, Mahamid J, Grigorieff N

eLife 10 (2021)

PMID: 34114559

Related EMDB entry:
Deposited:
2021-06-12
Released:
2021-11-15
Last modified:
2021-11-15
Imageset size:
67.79 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • reconstructed volumes
  • micrographs - single frame
  • tilt series
1. 50S templates of Mycoplasma pneumoniae used for 2D and 3D template matching
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
2
Image size:
(None, None)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(None, None)
Details:
3D density maps used as templates to detect 50S ribosomal subunits the 2D images and 3D tomograms of Mycoplasma pneumoniae cells by 2D and 3D template matching, respectively. The pixel size of the map used for 2D template matching is 1.27 Å. The map used for 3D template matching has a pixel size of 6.80 Å.
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2. Single-exposure micrographs of untilted Mycoplasma pneumoniae cells
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
19
Image size:
(1919, 1855)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.27, 1.27)
Details:
19 micrographs of Mycoplasma pneumoniae cells, using movies of 20 frames and a total exposures of 32 e-/Å^2. The movies were aligned using cisTEM, and exposure weighting was applied to calculate the frame average.
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3. Tomographic tilt series of Mycoplasma pneumoniae cells
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
19
Image size:
(3708, 3708)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.7, 1.7)
Details:
19 aligned tomographic tilt series of Mycoplasma pneumoniae cells collected using SerialEM. Tilts ranged from -60 to 60 deg using 3 deg angular steps. The total exposure was 129 e-/Å^2. The tilt series were recorded after a pre-exposure of 32 e-/Å^2 to collect a 2D image of each cell at higher resolution.
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4. Tomographic reconstructions of Mycoplasma pneumoniae cells
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
19
Image size:
(928, 928)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(6.8, 6.8)
Details:
19 tomographic reconstructions of Mycoplasma pneumoniae cells
Files:
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Files:
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