EMPIAR-10708 - Apoferritin, TMV and T20S proteasome in nanofluidic channels [multiple data sets in TIFF format]

EMPIAR-10708

Apoferritin, TMV and T20S proteasome in nanofluidic channels [multiple data sets in TIFF format]

Publication:

Nanofluidic chips for cryo-EM structure determination from picoliter sample volumes

Huber ST, Sarajlic E, Huijink R, Weis F, Evers WH, Jakobi AJ

eLife 11 (2022)

PMID: 35060902

DOI: 10.7554/elife.72629

Related EMDB entry:

EMD-12901 EMD-12915 EMD-12903

Deposited:

2021-05-07

Released:

2021-10-19

Last modified:

2022-02-28

Imageset size:

308.32 GB

Imageset DOI:

10.6019/EMPIAR-10708

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies of pyrococcus furiosus apoferritin in silicon nitride nanochannels

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

948

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.8127, 0.8127)

Details:

Movies are not gain-corrected
gain reference is in data/ApoFtn/CountRef_map_0044_001.mrc
needs to be flipped in y direction
defect file is in data/ApoFtn/defects_map_0044_001.txt
3.4 mg/mL concentration of sample
63 e-/A**2 total exposure

Files:

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2. Multi-frame movies of T20S proteasome in silicon nitride nanochannels

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

121

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.288, 1.288)

Details:

Gain-corrected compressed tif movies
1.4 mg/mL concentration of sample
59.7 e-/A**2 total exposure

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Multi-frame movies of TMV in silicon nitride nanochannels

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.288, 1.288)

Details:

Gain-corrected compressed tif movies
1.1 mg/mL concentration of sample
59.7 e-/A**2 total exposure

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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