EMPIAR-10708 - Apoferritin, TMV and T20S proteasome in nanofluidic channels [multiple data sets in TIFF format]

EMPIAR-10708

Apoferritin, TMV and T20S proteasome in nanofluidic channels [multiple data sets in TIFF format]

Publication:

Nanofluidic chips for cryo-EM structure determination from picoliter sample volumes

Huber ST, Sarajlic E, Huijink R, Weis F, Evers WH, Jakobi AJ

eLife 11 (2022)

PMID: 35060902

DOI: 10.7554/elife.72629

Related EMDB entry:

EMD-12901 EMD-12915 EMD-12903

Deposited:

2021-05-07

Released:

2021-10-19

Last modified:

2022-02-28

Imageset size:

308.32 GB

Imageset DOI:

10.6019/EMPIAR-10708

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies of pyrococcus furiosus apoferritin in silicon nitride nanochannels

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

948

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.8127, 0.8127)

Details:

Movies are not gain-corrected
gain reference is in data/ApoFtn/CountRef_map_0044_001.mrc
needs to be flipped in y direction
defect file is in data/ApoFtn/defects_map_0044_001.txt
3.4 mg/mL concentration of sample
63 e-/A**2 total exposure

Files:

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2. Multi-frame movies of T20S proteasome in silicon nitride nanochannels

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

121

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.288, 1.288)

Details:

Gain-corrected compressed tif movies
1.4 mg/mL concentration of sample
59.7 e-/A**2 total exposure

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Multi-frame movies of TMV in silicon nitride nanochannels

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.288, 1.288)

Details:

Gain-corrected compressed tif movies
1.1 mg/mL concentration of sample
59.7 e-/A**2 total exposure

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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