EMPIAR-10679 - Subtomograms of nucleosomes extracted from cryo-tomograms of Drosophila melanogaster embryos [1 subtomograms in EM format]

EMPIAR-10679

Subtomograms of nucleosomes extracted from cryo-tomograms of Drosophila melanogaster embryos [1 subtomograms in EM format]

Publication:

HEMNMA-3D: Cryo Electron Tomography Method Based on Normal Mode Analysis to Study Continuous Conformational Variability of Macromolecular Complexes

Harastani M, Eltsov M, Leforestier A, Jonic S

Frontiers in molecular biosciences 8 (2021)

PMID: 34095222

DOI: 10.3389/fmolb.2021.663121

Related EMDB entry:

EMD-12699

Deposited:

2021-03-31

Released:

2021-06-18

Last modified:

2021-06-18

Imageset size:

666.33 MB

Imageset DOI:

10.6019/EMPIAR-10679

Experimental metadata:

Download xml json

Contains:

subtomograms

Image Sets

1. Unaligned subtomograms of nucleosomes extracted from in situ cryo-tomograms of Drosophila melanogaster embryonic brain

Category:

subtomograms

Image format:

No. of images or tilt series:

Image size:

(None, None)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(4.4, 4.4)

Details:

Tilt series were recorded using the dose-symmetric recording scheme of Serial EM software at a nominal magnification of 64000 and a target defocus of -3.5 ˚ µm. The total dose was set to 91.5 e−/ A˚
A marker-less tilt series alignment was done in IMOD. Nucleosomes were manually picked in IMOD, and 64x64x64 voxel subtomograms (voxel size of 4.4 A) were extracted from the volumes.

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Citations

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Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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