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EMPIAR-10679
Subtomograms of nucleosomes extracted from cryo-tomograms of Drosophila melanogaster embryos [1 subtomograms in EM format]
Publication:

HEMNMA-3D: Cryo Electron Tomography Method Based on Normal Mode Analysis to Study Continuous Conformational Variability of Macromolecular Complexes

Harastani M, Eltsov M, Leforestier A, Jonic S

Frontiers in molecular biosciences 8 (2021)

PMID: 34095222

Related EMDB entry:
EMD-12699
Deposited:
2021-03-31
Released:
2021-06-18
Last modified:
2021-06-18
Imageset size:
666.33 MB
Imageset DOI:
10.6019/EMPIAR-10679
Experimental metadata:
Download xml json
Contains:
  • subtomograms
Image Sets
1. Unaligned subtomograms of nucleosomes extracted from in situ cryo-tomograms of Drosophila melanogaster embryonic brain
Category:
subtomograms
Image format:
EM
No. of images or tilt series:
1
Image size:
(None, None)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(4.4, 4.4)
Details:
Tilt series were recorded using the dose-symmetric recording scheme of Serial EM software at a nominal magnification of 64000 and a target defocus of -3.5 ˚ µm. The total dose was set to 91.5 e−/ A˚
A marker-less tilt series alignment was done in IMOD. Nucleosomes were manually picked in IMOD, and 64x64x64 voxel subtomograms (voxel size of 4.4 A) were extracted from the volumes.
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The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.
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