EMPIAR logo

Electron Microscopy Public Image Archive

EMPIAR-10613
DNA origami signposts for identifying proteins on cell membranes by electron cryotomography [2 tilt series in MRC format]
Publication:

DNA origami signposts for identifying proteins on cell membranes by electron cryotomography

Silvester E, Vollmer B, Pražák V, Vasishtan D, Machala EA, Whittle C, Black S, Bath J, Turberfield AJ, Grünewald K, Baker LA

Cell 184 (2021) 1110-1121.e16

PMID: 33606980

Related EMDB entry:
EMD-12188
Deposited:
2020-12-16
Released:
2021-04-14
Last modified:
2021-06-30
Imageset size:
40.64 MB
Imageset DOI:
10.6019/EMPIAR-10613
Experimental metadata:
Download xml json
Contains:
  • tilt series
Image Sets
1. Tomograms of sfGFP-glycoprotein B vesicles with DNA origami SPOTs
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
2
Image size:
(None, None)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(16.32, 16.32)
Details:
Tilt series were phase flipped and binned 2x with IMOD prior to reconstruction. Tomograms were then binned a further 3x and bandpass filtered between 2000 and 300 Å with Bsoft.
Files:
Loading...
Browse All Files
Quick Links
Browse EMPIAR
Sample Preparation Widget
Volume Browser
Tomo Viewer Prototype
Claim entries to your ORCiD
Talks and Tutorials
EMPIAR Quick tour
Publications
Re-use study
Statistics
EMPIAR in the news
Contact us
COVID-19 Data Portal
EMDB
BioImage Archive
PDBe
EMNavigator@PDBj
PDBj
Citations
High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles
Kim K, Li H, Clarke OB. (2025)
Self-supervised learning for generalizable particle picking in cryo-EM micrographs.
Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)
In situ structure determination of conformationally flexible targets using nextPYP.
Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)
CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.
Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)
Few-shot learning for non-vitrified ice segmentation.
Vivas-Lago A, Castaño-Díez D. (2025)
Automated removal of corrupted tilts in cryo-electron tomography.
Majtner T, Turoňová B. (2025)
Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.
Felix J, De Munck S, Bazan JF, Savvides SN. (2025)
Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.
Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)
Cryo-EM Structure Reconstruction by Gaussian Splatting: Pushing the Resolution to Extreme
Zeng B, Liu S, Cheng S, Xu G, Fan H. (2025)
Molecular exaptation by the integrin αI domain.
Hollis JA, Chan MC, Malik HS, Campbell MG. (2025)
See all citations
Protein Data Bank Japan News
[2025-09-17]
231 new PDB entries have been released on 2025-09-17.
[2025-07-17]
[wwPDB] Transitioning to PDBx/mmCIF and Extended PDB IDs
[2025-06-25]
[wwPDB] Extension to EMDB Accession Codes
[2025-04-18]
[wwPDB] Download Snapshots of the PDB Core Archive via HTTPS, SYNC, RSYNC, or FTP
[2025-03-25]
Announcement of the PDBj Newsletter Vol.25 release
[2025-03-11]
[wwPDB] Poster Prize Awarded at The Biophysical Society Meeting
[2025-03-10]
[wwPDB] New version of PDB-IHM validation reports now include assessments of crosslinking mass spectrometry-based structure models
[2025-01-30]
[wwPDB] Take the PDBx/mmCIF User Guide Survey and Win!
[2025-01-17]
[wwPDB] PDB-Dev now PDB-IHM
[2025-01-09]
[wwPDB] Time-stamped Copies of PDB and EMDB Archives
See all news