EMPIAR-10613 - DNA origami signposts for identifying proteins on cell membranes by electron cryotomography [2 tilt series in MRC format]

EMPIAR-10613

DNA origami signposts for identifying proteins on cell membranes by electron cryotomography [2 tilt series in MRC format]

Publication:

DNA origami signposts for identifying proteins on cell membranes by electron cryotomography

Silvester E, Vollmer B, Pražák V, Vasishtan D, Machala EA, Whittle C, Black S, Bath J, Turberfield AJ, Grünewald K, Baker LA

Cell 184 (2021) 1110-1121.e16

PMID: 33606980

DOI: 10.1016/j.cell.2021.01.033

Related EMDB entry:

EMD-12188

Deposited:

2020-12-16

Released:

2021-04-14

Last modified:

2021-06-30

Imageset size:

38.76 MB

Imageset DOI:

10.6019/EMPIAR-10613

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Tomograms of sfGFP-glycoprotein B vesicles with DNA origami SPOTs

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(None, None)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(16.32, 16.32)

Details:

Tilt series were phase flipped and binned 2x with IMOD prior to reconstruction. Tomograms were then binned a further 3x and bandpass filtered between 2000 and 300 Å with Bsoft.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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