EMPIAR-10613 - DNA origami signposts for identifying proteins on cell membranes by electron cryotomography [2 tilt series in MRC format]

EMPIAR-10613

DNA origami signposts for identifying proteins on cell membranes by electron cryotomography [2 tilt series in MRC format]

Publication:

DNA origami signposts for identifying proteins on cell membranes by electron cryotomography

Silvester E, Vollmer B, Pražák V, Vasishtan D, Machala EA, Whittle C, Black S, Bath J, Turberfield AJ, Grünewald K, Baker LA

Cell 184 (2021) 1110-1121.e16

PMID: 33606980

DOI: 10.1016/j.cell.2021.01.033

Related EMDB entry:

EMD-12188

Deposited:

2020-12-16

Released:

2021-04-14

Last modified:

2021-06-30

Imageset size:

38.76 MB

Imageset DOI:

10.6019/EMPIAR-10613

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Tomograms of sfGFP-glycoprotein B vesicles with DNA origami SPOTs

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(None, None)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(16.32, 16.32)

Details:

Tilt series were phase flipped and binned 2x with IMOD prior to reconstruction. Tomograms were then binned a further 3x and bandpass filtered between 2000 and 300 Å with Bsoft.

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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