EMPIAR-10600 - Human DPP9-CARD8 complex-S297A [3840 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-10600

Human DPP9-CARD8 complex-S297A [3840 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome formation by sequestering its active C-terminal fragment

Sharif H, Hollingsworth LR, Griswold AR, Hsiao JC, Wang Q, Bachovchin DA, Wu H

Immunity 54 (2021) 1392-1404.e10

PMID: 34019797

DOI: 10.1016/j.immuni.2021.04.024

Related EMDB entry:

EMD-22974

Deposited:

2020-12-16

Released:

2021-05-25

Last modified:

2022-02-15

Imageset size:

967.89 GB

Imageset DOI:

10.6019/EMPIAR-10600

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi frame micographs of CARD8-DPP9-S297A-TILT

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3840

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

For CARD8(S297A)-DPP9, 3,840 movies were collected at a stage tilt of 37° with defocus range between −0.8 to −2.5 μm and to record one shot for each of the four holes per stage movement through image shift. All movies were exposed with a total dose of 63.67 e-/Å2 for 1.5 s fractionated over 50 frames. References are in references folder

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

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