EMPIAR-10542
Serial electron diffraction from hen egg-white lysozyme [34 diffraction images in BIG DATA VIEWER HDF5 format]
Publication:

Serial protein crystallography in an electron microscope

Bücker R, Hogan-Lamarre P, Mehrabi P, Schulz EC, Bultema LA, Gevorkov Y, Brehm W, Yefanov O, Oberthür D, Kassier GH, Miller RJD

Nature communications 11 (2020) 996-1003

PMID: 32081905

Related EMDB entry:
Deposited:
2020-10-29
Released:
2021-08-13
Last modified:
2021-08-13
Imageset size:
17.70 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • diffraction images
1. Serial electron diffraction raw data from Lysozyme nano-crystals, taken with dose fractionation
Category:
diffraction images
Image format:
BIG DATA VIEWER HDF5
No. of images or tilt series:
34
Image size:
(1556, 516)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Diffraction data at 200 kV, 1580 mm calibrated camera length, 55 microns detector pixel size.
Dead-pixel mask and Gain reference in Ref12_pxmask.tif and Ref12_reference.tif
Stream files in streams/ are CrystFEL-format indexing solutions and integrated intensities and can be used for merging into a crystallographic data set.
To handle the files, diffractem is strongly suggested: github.com/robertbuecker/diffractem
Files:
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Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)