EMPIAR-10542 - Serial electron diffraction from hen egg-white lysozyme [34 diffraction images in BIG DATA VIEWER HDF5 format]

EMPIAR-10542

Serial electron diffraction from hen egg-white lysozyme [34 diffraction images in BIG DATA VIEWER HDF5 format]

Publication:

Serial protein crystallography in an electron microscope

Bücker R, Hogan-Lamarre P, Mehrabi P, Schulz EC, Bultema LA, Gevorkov Y, Brehm W, Yefanov O, Oberthür D, Kassier GH, Miller RJD

Nature communications 11 (2020) 996-1003

PMID: 32081905

DOI: 10.1038/s41467-020-14793-0

Related EMDB entry:

EMD-10090

Deposited:

2020-10-29

Released:

2021-08-13

Last modified:

2021-08-13

Imageset size:

17.70 GB

Imageset DOI:

10.6019/EMPIAR-10542

Experimental metadata:

Download xml json

Contains:

diffraction images

Image Sets

1. Serial electron diffraction raw data from Lysozyme nano-crystals, taken with dose fractionation

Category:

diffraction images

Image format:

BIG DATA VIEWER HDF5

No. of images or tilt series:

Image size:

(1556, 516)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(None, None)

Details:

Diffraction data at 200 kV, 1580 mm calibrated camera length, 55 microns detector pixel size.
Dead-pixel mask and Gain reference in Ref12_pxmask.tif and Ref12_reference.tif
Stream files in streams/ are CrystFEL-format indexing solutions and integrated intensities and can be used for merging into a crystallographic data set.
To handle the files, diffractem is strongly suggested: github.com/robertbuecker/diffractem

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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