EMPIAR-10434
TEM tomograms of Drosophila tracheal terminal cells during subcellular tube formation [multiple data sets in TIFF and MRC formats]
Publication:

Transcytosis via the late endocytic pathway as a cell morphogenetic mechanism

Mathew R, Rios-Barrera LD, Machado P, Schwab Y, Leptin M

The EMBO journal (2020)

PMID: 32657472

Related EMDB entry:
Deposited:
2020-06-10
Released:
2020-08-12
Last modified:
2020-08-12
Imageset size:
366.68 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Aligned EM tomographs covering an embryonic tracheal terminal cell, overview image
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(25.49, 25.49)
Details:
Tilt series were performed in 1 degree increments from 60 to -60 degrees with 2.549 nm pixel size on a FEI Tecnai F30 electron microscope with a Gatan OneView camera. The serial tomograms were reconstructed and aligned using the IMOD package.
Files:
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2. Aligned EM tomographs covering an embryonic tracheal terminal cell, overview image
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(25.49, 25.49)
Details:
Tilt series were performed in 1 degree increments from 60 to -60 degrees with 2.549 nm pixel size on a FEI Tecnai F30 electron microscope with a Gatan OneView camera. The serial tomograms were reconstructed and aligned using the IMOD package.
Files:
Loading...
3. Aligned EM tomographs covering an embryonic tracheal terminal cell after 1 hour of dynamin inactivation
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(25.49, 25.49)
Details:
Tilt series were performed in 1 degree increments from 60 to -60 degrees with 2.549 nm pixel size on a FEI Tecnai F30 electron microscope with a Gatan OneView camera. The serial tomograms were reconstructed and aligned using the IMOD package.
Files:
Loading...
4. Aligned EM tomographs covering an embryonic tracheal terminal cell after 1 hour of dynamin inactivation
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(25.49, 25.49)
Details:
Tilt series were performed in 1 degree increments from 60 to -60 degrees with 2.549 nm pixel size on a FEI Tecnai F30 electron microscope with a Gatan OneView camera. The serial tomograms were reconstructed and aligned using the IMOD package.
Files:
Loading...
5. Tomographs covering subpositions within an embryonic tracheal terminal cell
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
45
Image size:
(4096, 4096)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(7.8, 7.8)
Details:
Data is organized by grids (g*) where the physical sections (s*) were present; hm* refers to the number of high-magnification images obtained from a given section. Tomograms are .mrc files, saved as .rec files. 3D reconstruction models are saved in the same folder as .mod and are also .mrc files generated using IMOD.
Files:
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6. Tomographs covering subpositions within an embryonic tracheal terminal cell
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
36
Image size:
(4096, 4096)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(7.8, 7.8)
Details:
Data is organized by grids (g*) where the physical sections (s*) were present; hm* refers to the number of high-magnification images obtained from a given section. Tomograms are .mrc files, saved as .rec files. 3D reconstruction models are saved in the same folder as .mod and are also .mrc files generated using IMOD.
Files:
Loading...
Files:
Loading...

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