EMPIAR-10345 - CryoEM dataset containing multiple conformations of the asymmetric αVβ8 integrin bound to two Fabs on a holey carbon grid (minimal preferred orientations) [multiple data sets in TIFF, MRC and MRCS formats]

EMPIAR-10345

CryoEM dataset containing multiple conformations of the asymmetric αVβ8 integrin bound to two Fabs on a holey carbon grid (minimal preferred orientations) [multiple data sets in TIFF, MRC and MRCS formats]

Publication:

Cryo-EM Reveals Integrin-Mediated TGF-β Activation without Release from Latent TGF-β

Campbell MG, Cormier A, Ito S, Seed RI, Bondesson AJ, Lou J, Marks JD, Baron J, Cheng Y, Nishimura SL

Cell (2020)

PMID: 31955848

DOI: 10.1016/j.cell.2019.12.030

Related EMDB entry:

EMD-20795

Deposited:

2019-12-13

Released:

2020-01-31

Last modified:

2020-02-07

Imageset size:

882.09 GB

Imageset DOI:

10.6019/EMPIAR-10345

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame
picked particles - single frame - processed

Image Sets

1. Unaligned 60-frame movies of αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1644

Image size:

(7676, 7420)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.6725, 0.6725)

Details:

αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid (particles have minimal preferred orientations): 1644 unaligned uncorrected movies were recorded automatically using SerialEM on a K2 camera in super-resolution (dstep=2.5) mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. The 12 second movies contain 60 frames and the super resolution pixel size is 0.6725 Å/px. The total dose per movie is ~55 e/Å^2. The gain reference anda file containing coordinates for a single dead column.

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2. Dose-weighted aligned micrographs of αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

1644

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.345, 1.345)

Details:

αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid (particles have minimal preferred orientations): 1644 aligned and dose-weighted (in MotionCor2) micrographs recorded automatically using SerialEM on a K2 camera (dstep=5) in super-resolution mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.345. The total dose per micrograph is ~55 e/Å^2.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Dose-weighted aligned particle stacks of αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

1642

Image size:

(300, 300)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.345, 1.345)

Details:

αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid (particles have minimal preferred orientations): Stacks of 84,266 dose-weighted (in MotionCor2) particles contributing to the seven conformational snapshots described in the publication . Particles were selected after 2D classification. Images were recorded automatically using SerialEM on a K2 camera (dstep=5) in super-resolution mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.345.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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