EMPIAR-10345
CryoEM dataset containing multiple conformations of the asymmetric αVβ8 integrin bound to two Fabs on a holey carbon grid (minimal preferred orientations) [multiple data sets in TIFF, MRC and MRCS formats]
Publication:

Cryo-EM Reveals Integrin-Mediated TGF-β Activation without Release from Latent TGF-β

Campbell MG, Cormier A, Ito S, Seed RI, Bondesson AJ, Lou J, Marks JD, Baron J, Cheng Y, Nishimura SL

Cell (2020)

PMID: 31955848

Related EMDB entry:
Deposited:
2019-12-13
Released:
2020-01-31
Last modified:
2020-02-07
Imageset size:
882.09 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
  • picked particles - single frame - processed
1. Unaligned 60-frame movies of αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1644
Image size:
(7676, 7420)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.6725, 0.6725)
Details:
αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid (particles have minimal preferred orientations): 1644 unaligned uncorrected movies were recorded automatically using SerialEM on a K2 camera in super-resolution (dstep=2.5) mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. The 12 second movies contain 60 frames and the super resolution pixel size is 0.6725 Å/px. The total dose per movie is ~55 e/Å^2. The gain reference anda file containing coordinates for a single dead column.
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2. Dose-weighted aligned micrographs of αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
1644
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.345, 1.345)
Details:
αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid (particles have minimal preferred orientations): 1644 aligned and dose-weighted (in MotionCor2) micrographs recorded automatically using SerialEM on a K2 camera (dstep=5) in super-resolution mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.345. The total dose per micrograph is ~55 e/Å^2.
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3. Dose-weighted aligned particle stacks of αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
1642
Image size:
(300, 300)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.345, 1.345)
Details:
αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid (particles have minimal preferred orientations): Stacks of 84,266 dose-weighted (in MotionCor2) particles contributing to the seven conformational snapshots described in the publication . Particles were selected after 2D classification. Images were recorded automatically using SerialEM on a K2 camera (dstep=5) in super-resolution mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.345.
Files:
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