CryoEM dataset containing multiple conformations of the asymmetric αVβ8 integrin bound to two Fabs on a holey carbon grid (minimal preferred orientations) [multiple data sets in TIFF, MRC and MRCS formats]
Publication:
Cryo-EM Reveals Integrin-Mediated TGF-β Activation without Release from Latent TGF-β
αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid (particles have minimal preferred orientations): 1644 unaligned uncorrected movies were recorded automatically using SerialEM on a K2 camera in super-resolution (dstep=2.5) mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. The 12 second movies contain 60 frames and the super resolution pixel size is 0.6725 Å/px. The total dose per movie is ~55 e/Å^2. The gain reference anda file containing coordinates for a single dead column.
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αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid (particles have minimal preferred orientations): 1644 aligned and dose-weighted (in MotionCor2) micrographs recorded automatically using SerialEM on a K2 camera (dstep=5) in super-resolution mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.345. The total dose per micrograph is ~55 e/Å^2.
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αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid (particles have minimal preferred orientations): Stacks of 84,266 dose-weighted (in MotionCor2) particles contributing to the seven conformational snapshots described in the publication . Particles were selected after 2D classification. Images were recorded automatically using SerialEM on a K2 camera (dstep=5) in super-resolution mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.345.
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Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
Download a list of selected files.
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Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
Download a list of selected files.
It is possible to download files by specifying the file list with rsync command, etc.