EMPIAR-10333 - Single Particle Cryo-EM Reconstructions of Human FACT in Complex with Partially Assembled Sub-nucleosomes [multiple data sets in TIFF, MRC and MRCS formats]

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EMPIAR-10333

Single Particle Cryo-EM Reconstructions of Human FACT in Complex with Partially Assembled Sub-nucleosomes [multiple data sets in TIFF, MRC and MRCS formats]

Publication:

FACT caught in the act of manipulating the nucleosome

Liu YL, Zhou KZ, Zhang NZ, Wei HW, Tan YZT, Zhang ZZ, Carragher BC, Potter CSP, D'Arcy SD, Luger KL

Nature (2019)

PMID: 31775157

DOI: 10.1038/s41586-019-1820-0

Related EMDB entry:

EMD-20841 EMD-20840

Deposited:

2019-10-21

Released:

2019-12-20

Last modified:

2021-11-08

Imageset size:

2.21 TB

Imageset DOI:

10.6019/EMPIAR-10333

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame
picked particles - single frame - processed

Image Sets

1. Unaligned multi-frame micrographs

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

8319

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.0961, 1.0961)

Details:

Unaligned movies saved in TIF format. The first data collection (19feb16b) was performed using a dose of ~66.37 e-/Å2 across 50 frames (200 msec per frame) at a dose rate of ~8.0 e–/pix/sec, using a set defocus range of -1.9 μm to -2.0 μm. The second data collection (19may31e) was performed using a dose of ~ 63.61 e-/Å2 across 50 frames (200 msec per frame) at a dose rate of ~7.6 e–/pix/sec, using a set defocus range of -1.5 μm to -2.5 μm. The gain reference images for each data collection are in the respective references folder.

Files:

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2. Aligned and dose-weighted micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

6934

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.0961, 1.0961)

Details:

Aligned and dose-weighted micrographs. Motion correction was performed using Relion 3.

Files:

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3. Final Particle Stacks for Class 1 and 2 with Euler Angles and Shifts

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

Image size:

(200, 200)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.403008, 1.403008)

Details:

Final particle stacks for both class 1 and 2 maps with corresponding Relion .star file containing the refined Euler angles and shifts.

Files:

This entry cannot be downloaded from PDBj.

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Citations

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Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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