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EMPIAR-10333
Single Particle Cryo-EM Reconstructions of Human FACT in Complex with Partially Assembled Sub-nucleosomes [multiple data sets in TIFF, MRC and MRCS formats]
Publication:
FACT caught in the act of manipulating the nucleosome
Unaligned movies saved in TIF format. The first data collection (19feb16b) was performed using a dose of ~66.37 e-/Å2 across 50 frames (200 msec per frame) at a dose rate of ~8.0 e–/pix/sec, using a set defocus range of -1.9 μm to -2.0 μm. The second data collection (19may31e) was performed using a dose of ~ 63.61 e-/Å2 across 50 frames (200 msec per frame) at a dose rate of ~7.6 e–/pix/sec, using a set defocus range of -1.5 μm to -2.5 μm. The gain reference images for each data collection are in the respective references folder.
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Aligned and dose-weighted micrographs. Motion correction was performed using Relion 3.
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Final particle stacks for both class 1 and 2 maps with corresponding Relion .star file containing the refined Euler angles and shifts.
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