EMPIAR-10203 - Structure of the Macrobrachium rosenbergii Nodavirus: A new genus within the Nodaviridae? [2459 micrographs in MRC format]

EMPIAR-10203

Structure of the Macrobrachium rosenbergii Nodavirus: A new genus within the Nodaviridae? [2459 micrographs in MRC format]

Publication:

Structure of the Macrobrachium rosenbergii nodavirus: A new genus within the Nodaviridae?

Ho KL, Gabrielsen M, Beh PL, Kueh CL, Thong QX, Streetley J, Tan WS, Bhella D

PLoS biology 16 (2018)

PMID: 30346944

DOI: 10.1371/journal.pbio.3000038

Related EMDB entry:

EMD-0129

Deposited:

2018-08-02

Released:

2018-10-25

Last modified:

2022-02-08

Imageset size:

130.44 GB

Imageset DOI:

10.6019/EMPIAR-10203

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Motion corrected micrographs of Macrobrachium rosenbergii nodavirus virus-like particles

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

2459

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.06, 1.06)

Details:

To collect high-resolution data on MrNV VLPs, grids were imaged at the electron bioimaging centre (eBIC), Diamond Light Source (UK) using an FEI Titan Krios operated at 47,170 X magnification. Images were recorded on a Gatan K2 BioQuantum energy-filtered direct detector camera operated in zero-loss imaging mode with a slit width of 20 eV. Five second exposures were recorded in electron counting mode, at a frame-rate of four frames per second and a dose rate of 1.8 electrons/pixel/frame. The pixel size was 1.06 Å/pixel.
Motion correction was performed in Motioncor2.

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