To collect high-resolution data on MrNV VLPs, grids were imaged at the electron bioimaging centre (eBIC), Diamond Light Source (UK) using an FEI Titan Krios operated at 47,170 X magnification. Images were recorded on a Gatan K2 BioQuantum energy-filtered direct detector camera operated in zero-loss imaging mode with a slit width of 20 eV. Five second exposures were recorded in electron counting mode, at a frame-rate of four frames per second and a dose rate of 1.8 electrons/pixel/frame. The pixel size was 1.06 Å/pixel. Motion correction was performed in Motioncor2.