EMPIAR-12723 - Single-particle cryo-EM of Staphylococcus aureus ribosomes inhibited by fusidic acid and fusidic acid cyclopentane [multiple data sets in EER, TIFF and MRC formats]

EMPIAR-12723

Single-particle cryo-EM of Staphylococcus aureus ribosomes inhibited by fusidic acid and fusidic acid cyclopentane [multiple data sets in EER, TIFF and MRC formats]

Publication:

Structures of the Staphylococcus aureus ribosome inhibited by fusidic acid and fusidic acid cyclopentane

González-López A, Larsson DSD, Koripella RK, Cain BN, Chavez MG, Hergenrother PJ, Sanyal S, Selmer M

Scientific reports 14 (2024)

PMID: 38902339

DOI: 10.1038/s41598-024-64868-x

Related EMDB entry:

EMD-17364 EMD-17365 EMD-17363

Deposited:

2025-04-04

Released:

2025-05-14

Last modified:

2025-05-14

Imageset size:

11.05 TB

Imageset DOI:

10.6019/EMPIAR-12723

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - single frame - processed

Image Sets

1. Unaligned multi-frame micrographs of FA-CP inhibited ribosomes

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

17252

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.728, 0.728)

Details:

Data (raw movies) were collected at SciLifeLab in Umeå, Sweden in a Titan Krios G2 operated at 300 kV and equipped with a Falcon-4i direct electron detector and Selectris energy filter using 10 eV slit. The data were acquired at 165,000 x nominal magnification with a calibrated pixel size of 0.728 Å. A 50 µm C2 aperture was inserted and the TEM was operated in nanoprobe mode at spot size 6 and 520 nm beam size. The EER formatted movies were collected in 684 raw frames with a total dose of 27.77 e-/Å2 (12.45 e-/pixel/s) over 2.23 s with a set defocus between -0.7 to -1.3 µm.
The movies are not gain-corrected, use the file provided within the main folder.

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2. Unaligned multi-frame micrographs of FA inhibited ribosomes

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5486

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.824, 0.824)

Details:

Data (raw movies) were collected at SciLifeLab in Solna, Sweden on a Titan Krios G2 operated at 300 kV and equipped with a K3 BioQuantum direct electron detector and energy filter using 20 eV slit. The data were acquired at 105,000 x nominal magnification with a calibrated pixel size of 0.824 Å. A 50 µm C2 aperture was inserted and the TEM was operated in nanoprobe mode at spot size 6 and 670 nm beam size. The movies were collected in 30 frames over 1.3 s with a total dose of 30.83 e-/Å2 (16.1 e-/pixel/s) with a set defocus between -0.5 to -1.5 µm.
The movies are gain-corrected.

Files:

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3. EMD-17364 particles (FA-CP CHI)

Category:

picked particles - single frame - processed

Image format:

MRC

No. of images or tilt series:

303005

Image size:

(700, 700)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.728, 0.728)

Details:

---

Files:

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4. EMD-17363 particles (FA-CP POST)

Category:

picked particles - single frame - processed

Image format:

MRC

No. of images or tilt series:

40800

Image size:

(700, 700)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.728, 0.728)

Details:

---

Files:

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5. EMD-17365 particles (FA CHI)

Category:

picked particles - single frame - processed

Image format:

MRC

No. of images or tilt series:

178679

Image size:

(600, 600)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.824, 0.824)

Details:

---

Files:

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6. Unaligned multi-frame micrographs of FA-CP inhibited ribosomes screening Glacios data

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6052

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.952, 0.952)

Details:

Raw movies from the FA-CP inhibited ribosomes screening in a Glacios microscope operated at 200 kV with a magnification of 150,000x with a pixel size of 0.952 Å/pix. The movies were collected in 31 frames over 28.74 s with a total dose of 29.8 e-/Å2 (0.94 e-/pixel/s) with a set defocus between -0.5 to -1.5 µm.
The movies are gain corrected.

Files:

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7. Unaligned multi-frame micrographs of FA inhibited ribosomes screening Glacios data

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1460

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.952, 0.952)

Details:

Raw movies from the FA inhibited ribosomes screening in a Glacios microscope operated at 200 kV with a magnification of 150,000x with a pixel size of 0.952 Å/pix. The movies were collected in 30 frames over 27.79 s with a total dose of 30 e-/Å2 (1.00 e-/pixel/s) with a set defocus between -0.5 to -1.5 µm.
The movies are gain corrected.

Files:

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