EMPIAR-12612 - Molecular architecture of thylakoid membranes within intact spinach chloroplasts [multiple data sets in TIFF and MRC formats]

EMPIAR-12612

Molecular architecture of thylakoid membranes within intact spinach chloroplasts [multiple data sets in TIFF and MRC formats]

Publication:

Molecular architecture of thylakoid membranes within intact spinach chloroplasts

Wietrzynski W, Lamm L, Wood WHJ, Loukeri MJ, Malone L, Peng T, Johnson MP, Engel BD

bioRxiv (2024)

DOI: 10.1101/2024.11.24.625035

Related EMDB entry:

EMD-52542 EMD-52545 EMD-52548 EMD-52546 EMD-52543 EMD-52547 EMD-52544

Deposited:

2024-11-21

Released:

2025-05-09

Last modified:

2025-05-09

Imageset size:

303.89 GB

Imageset DOI:

10.6019/EMPIAR-12612

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
reconstructed volumes

Image Sets

1. Spinacia oleracea, isolated chloroplast; Cryo-ET sessions; Unaligned frames and .mdocs

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2028

Image size:

(3838, 3710)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(3.52, 3.52)

Details:

Directory contains raw frames and .mdoc files corresponding to 30+ tomograms acquired over multiple sessions and selected for quality. Collected on Titan G1 with Bioquantum energy filter and K2 Summit detector.

Files:

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2. Spinacia oleracea, isolated chloroplasts; Denoised tomograms

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

Image size:

(928, 928)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(14.08, 14.08)

Details:

Tomograms of isolated Spinach chloroplasts denoised using CryoCARE from odd- and even-frames.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

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3. Spinacia oleracea, isolated chloroplasts; Membrane segmentations

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

Image size:

(928, 928)

Pixel type:

SIGNED BYTE

Pixel spacing:

(14.08, 14.08)

Details:

Selected segmentations of tomograms of isolated chloroplasts from Spinach. Membranes present in the volumes were detected and segmented using MemBrain-seg program.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

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