EMPIAR-12501 - PSI-LHCI supercomplex binding with Lhcas from C. subellipsoidea [multiple data sets in EER and MRC formats]

EMPIAR-12501

PSI-LHCI supercomplex binding with Lhcas from C. subellipsoidea [multiple data sets in EER and MRC formats]

Publication:

Structural study of the chlorophyll between Lhca8 and PsaJ in an Antarctica green algal photosystem I-LHCI supercomplex revealed by its atomic structure

Tsai PC, Kato K, Shen JR, Akita F

Biochimica et biophysica acta. Bioenergetics 1866 (2025)

PMID: 39947506

DOI: 10.1016/j.bbabio.2025.149543

Related EMDB entry:

EMD-62512 EMD-62511

Deposited:

2024-11-29

Released:

2025-06-27

Last modified:

2025-06-27

Imageset size:

7.95 TB

Imageset DOI:

10.6019/EMPIAR-12501

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. Unaligned multi-frame micrographs of photosystem I- LHCI supercomplex

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

14766

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.727, 0.727)

Details:

Raw, non aligned micrograph movies in EER format from Falcon 4i detector.Total electron dose for 3.42 s exposure is 50 electrons per square angstrom, which is fractionated into 1240 EER frames. This data set was collected from 1 grid and the nominal defocus range is -0.8 to -2.0 μm.
Acceleration Voltage: 300 kV, Cs: 2.7 mm, Magnification: 165,000, Pixel Size: 0.727 angstrom/pixel.

Files:

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2. Motion-corrected and dose-weighted micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

14766

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.727, 0.727)

Details:

CryoSPARC job folder of the patch Motion correction.

Files:

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3. patch CTF estimation treated micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

14766

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.727, 0.727)

Details:

CryoSPARC job folder of the patch CTF estimation.

Files:

This entry cannot be downloaded from PDBj.

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We recommend using rsync, aspera, globus, etc.

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4. Gain reference file

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(None, None)

Details:

Gain reference file for motion correction.

Files:

This entry cannot be downloaded from PDBj.

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Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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