EMPIAR-12421 - Pre-turnover condition dataset for Bacillus subtilis class Ib ribonucleotide reductase [3476 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-12421

Pre-turnover condition dataset for Bacillus subtilis class Ib ribonucleotide reductase [3476 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Conformational Landscapes of a Class I Ribonucleotide Reductase Complex during Turnover Reveal Intrinsic Dynamics and Asymmetry

Xu D, Thomas WC, Burnim AA, Ando N

(---)

Related EMDB entry:

EMD-44991 EMD-44992 EMD-44985 EMD-44947 EMD-45000 EMD-44995 EMD-44999

Deposited:

2024-10-11

Released:

2025-03-28

Last modified:

2025-03-28

Imageset size:

1.08 TB

Imageset DOI:

10.6019/EMPIAR-12421

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Raw movies for Bacillus subtilis class Ib RNR pre-turnover condition

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3476

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.022, 1.022)

Details:

Raw movies collected with 50 frames, 50 e- total dose, 2.031 s exposure time, 0.0405 s frame time, and 24.77 e- Å-2 s-1 dose rate. Polished particles are in the the Polish folder. Information of particles used in consensus reconstruction is available as consensus_preturnover.star . Information of particles used in cryoDRGN analysis is available as cryoDRGN_preturnover.star .

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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