EMPIAR-12421 - Pre-turnover condition dataset for Bacillus subtilis class Ib ribonucleotide reductase [3476 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-12421

Pre-turnover condition dataset for Bacillus subtilis class Ib ribonucleotide reductase [3476 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Conformational Landscapes of a Class I Ribonucleotide Reductase Complex during Turnover Reveal Intrinsic Dynamics and Asymmetry

Xu D, Thomas WC, Burnim AA, Ando N

(---)

Related EMDB entry:

EMD-44991 EMD-44992 EMD-44985 EMD-44947 EMD-45000 EMD-44995 EMD-44999

Deposited:

2024-10-11

Released:

2025-03-28

Last modified:

2025-03-28

Imageset size:

1.08 TB

Imageset DOI:

10.6019/EMPIAR-12421

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Raw movies for Bacillus subtilis class Ib RNR pre-turnover condition

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3476

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.022, 1.022)

Details:

Raw movies collected with 50 frames, 50 e- total dose, 2.031 s exposure time, 0.0405 s frame time, and 24.77 e- Å-2 s-1 dose rate. Polished particles are in the the Polish folder. Information of particles used in consensus reconstruction is available as consensus_preturnover.star . Information of particles used in cryoDRGN analysis is available as cryoDRGN_preturnover.star .

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Citations

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Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)