EMPIAR-11970 - Single particle cryoEM dataset of nucleosomes containing H2AK119ub [12211 multi-frame micrographs composed of 199 frames each in EER format]

EMPIAR-11970

Single particle cryoEM dataset of nucleosomes containing H2AK119ub [12211 multi-frame micrographs composed of 199 frames each in EER format]

Publication:

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch

Hicks CW, Wolberger C

(---)

Related EMDB entry:

EMD-29781 EMD-29778

Deposited:

2024-01-19

Released:

2025-07-10

Last modified:

2025-07-10

Imageset size:

7.30 TB

Imageset DOI:

10.6019/EMPIAR-11970

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of H2AK119ub-modified nucleosome

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

12211

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.97, 0.97)

Details:

The gain reference file generated on 20220902 should be used with the raw data collected on 20220910, while the gain reference file generated on 20220919 should be used with the raw data collected on 20220925.

Files:

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)

Few-shot learning for non-vitrified ice segmentation.

Vivas-Lago A, Castaño-Díez D. (2025)

Automated removal of corrupted tilts in cryo-electron tomography.

Majtner T, Turoňová B. (2025)

Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)

Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.

Felix J, De Munck S, Bazan JF, Savvides SN. (2025)

See all citations

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