EMPIAR-11783 - SpCas9 bound to 18 nucleotide complementary DNA substrate in the catalytic state [multiple data sets in TIFF and DM4 formats]

EMPIAR-11783

SpCas9 bound to 18 nucleotide complementary DNA substrate in the catalytic state [multiple data sets in TIFF and DM4 formats]

Publication:

R-loop formation and conformational activation mechanisms of Cas9

Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M

Nature 609 (2022) 191-196

PMID: 36002571

DOI: 10.1038/s41586-022-05114-0

Related EMDB entry:

EMD-14499

Deposited:

2023-11-10

Released:

2023-12-11

Last modified:

2023-12-11

Imageset size:

1.76 TB

Imageset DOI:

10.6019/EMPIAR-11783

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. SpCas9 bound to 18 nucleotide complementary DNA substrate in the catalytic state

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

10547

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.65, 0.65)

Details:

Unaligned multi-frame movies with each containing 47 frames per movie collected at 300 kV in super-resolution counting mode. Total accumulated electron exposure on the specimen level corresponds to 64.2 electrons per Å^2. Movies are not gain corrected and the gain reference is provided. Data acquired with EPU.

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2. Gain reference

Category:

micrographs - single frame

Image format:

DM4

No. of images or tilt series:

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.65, 0.65)

Details:

Gain reference

Files:

This entry cannot be downloaded from PDBj.

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We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

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