EMPIAR-11687
Cryo electron tomography of Cytochalasin D-induced protrusions of Drosophila S2 cells treated with thapsigargin or MG132 [multiple data sets in TIFF and MRC formats]
Publication:

CryoET shows cofilactin filaments inside the microtubule lumen

Ventura Santos C, Carter AP, Rogers SL

EMBO Reports (2023)

PMID: 37702953

Related EMDB entry:
Deposited:
2023-09-18
Released:
2023-10-03
Last modified:
2023-10-03
Imageset size:
107.59 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • reconstructed volumes
1. Raw multi-frame tilt images of Cytochalasin D and thapsigargin or MG132 treated protrusions of Drosophila S2 cells
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
13
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.329, 1.329)
Details:
This folder contains raw, multi-frame tilt images. Data was collected on a K3 detector in super-resolution mode with a super-resolution pixel size of 1.329Ã…/pixel. Mdoc files and the gain reference are provided inside the folder.
This data is from the preliminary experiment shown in Fig. EV2A of the associated publication. TS_167, TS_169, TS_171, TS_172, TS_173 are from cells treated with 2.5uM Cytochalasin D (2h) and 25uM MG132 (5h).
TS_178, TS_179, TS_180, TS_181, TS_182, TS_183, TS_185, TS_186 are from cells treated with 2.5uM Cytochalasin D (2h) and 2uM thapsigargin (5h).
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2. Binned, deconvolved tomograms of Cytochalasin D and thapsigargin or MG132 treated protrusions of Drosophila S2 cells
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
13
Image size:
(1022, 1440)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(10.74, 10.74)
Details:
Tomograms binned by 4 (from non-super resolution pixel size), reconstructed and deconvolved in WARP.
This data is from the preliminary experiment shown in Fig. EV2A of the associated publication. TS_167, TS_169, TS_171, TS_172, TS_173 are from cells treated with 2.5uM Cytochalasin D (2h) and 25uM MG132 (5h).
TS_178, TS_179, TS_180, TS_181, TS_182, TS_183, TS_185, TS_186 are from cells treated with 2.5uM Cytochalasin D (2h) and 2uM thapsigargin (5h).
Files:
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