EMPIAR-11672 - Single particle cryo-EM dataset of mitochondrial respiratory complex I from Macaca mulatta [2523 multi-frame micrographs composed of 40 frames each in MRC format]

EMPIAR-11672

Single particle cryo-EM dataset of mitochondrial respiratory complex I from Macaca mulatta [2523 multi-frame micrographs composed of 40 frames each in MRC format]

Publication:

Mammalian Respiratory Complex I Through the Lens of Cryo-EM

Agip AA, Blaza JN, Fedor JG, Hirst J

Annual review of biophysics 48 (2019) 165-184

PMID: 30786232

DOI: 10.1146/annurev-biophys-052118-115704

Related EMDB entry:

EMD-0151

Deposited:

2023-08-02

Released:

2023-10-03

Last modified:

2023-10-03

Imageset size:

1.50 TB

Imageset DOI:

10.6019/EMPIAR-11672

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (FoilHole*.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   3,307,272,031 kilobytes
Compressed size: 1,610,700,488 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Unaligned, gain-corrected micrographs of mitochondrial complex I from Macaca mulatta

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

2523

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.39, 1.39)

Details:

The micrographs are the average of 40 unaligned, gain-corrected frames.The dose rate was 25 electrons Å-2 s-1 with 2 s exposure, giving a total dose of 50 e Å-2.

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

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