EMPIAR-11653 - Single particle cryo-EM dataset of bovine complex I in the deactive state [2988 multi-frame micrographs composed of 20 frames each in MRC format]

EMPIAR-11653

Single particle cryo-EM dataset of bovine complex I in the deactive state [2988 multi-frame micrographs composed of 20 frames each in MRC format]

Publication:

Structure of the Deactive State of Mammalian Respiratory Complex I

Blaza JN, Vinothkumar KR, Hirst J

Structure (London, England : 1993) 26 (2018) 312-319000.0

PMID: 29395787

DOI: 10.1016/j.str.2017.12.014

Related EMDB entry:

EMD-3731

Deposited:

2023-08-10

Released:

2023-10-03

Last modified:

2023-10-03

Imageset size:

965.82 GB

Imageset DOI:

10.6019/EMPIAR-11653

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (FoilHole*.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   1,955,357,100 kilobytes
Compressed size: 1,012,676,570 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Unaligned, gain-corrected micrographs of mitochondrial complex I from Bos taurus in the deactive state

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

2988

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.38, 1.38)

Details:

The final particle positions are in columns 11 (X) and 12 (Y) of the shiny.star file. Micrographs are the average of 20 unaligned, gain-corrected frames. Data were collected over 2.5 seconds with a dose rate of 32 e / A-2 / s with a total dose of 80 e/A-2.

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

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