EMPIAR-11265 - Cryo-EM structures of Ib-pore and Ia-bound Ib-pore [multiple data sets in TIFF format]

EMPIAR-11265

Cryo-EM structures of Ib-pore and Ia-bound Ib-pore [multiple data sets in TIFF format]

Publication:

Cryo-EM structures reveal translocational unfolding in the clostridial binary iota toxin complex

Yamada T, Yoshida T, Kawamoto A, Mitsuoka K, Iwasaki K, Tsuge H

Nature structural & molecular biology 27 (2020) 288-296

PMID: 32123390

DOI: 10.1038/s41594-020-0388-6

Related EMDB entry:

EMD-0720 EMD-0721 EMD-0713

Deposited:

2022-09-22

Released:

2022-11-14

Last modified:

2022-11-14

Imageset size:

8.04 TB

Imageset DOI:

10.6019/EMPIAR-11265

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. Ib-pore

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2120

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.159, 1.159)

Details:

HT: 300 kV
Cs: 0.01 mm (corrected by Cs corrector)
Pixel size: 1.13 A/pix
Dose per frame: 0.4629 electron/angstrom^2
Defocus Range: -1.0 μm to -2.75 μm.

NB: The pixel size in the TIFF header is not correct.

Files:

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2. Ia-bound Ib-pore

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2151

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.159, 1.159)

Details:

HT: 300 kV
Cs: 0.01 mm (corrected by Cs corrector)
Pixel size: 1.13 A/pix
Dose per frame: 0.4629 electron/angstrom^2
Defocus Range: -0.8 μm to -2.5 μm.

NB: The pixel size in the TIFF header is not correct.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

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