EMPIAR-11265
Cryo-EM structures of Ib-pore and Ia-bound Ib-pore [multiple data sets in TIFF format]
Publication:

Cryo-EM structures reveal translocational unfolding in the clostridial binary iota toxin complex

Yamada T, Yoshida T, Kawamoto A, Mitsuoka K, Iwasaki K, Tsuge H

Nature structural & molecular biology 27 (2020) 288-296

PMID: 32123390

Related EMDB entry:
Deposited:
2022-09-22
Released:
2022-11-14
Last modified:
2022-11-14
Imageset size:
8.04 TB
Imageset DOI:
Experimental metadata:
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Deposited via PDBj
Contains:
  • micrographs - multiframe
1. Ib-pore
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2120
Image size:
(4096, 4096)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(1.159, 1.159)
Details:
HT: 300 kV
Cs: 0.01 mm (corrected by Cs corrector)
Pixel size: 1.13 A/pix
Dose per frame: 0.4629 electron/angstrom^2
Defocus Range: -1.0 μm to -2.75 μm.

NB: The pixel size in the TIFF header is not correct.
Files:
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2. Ia-bound Ib-pore
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2151
Image size:
(4096, 4096)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(1.159, 1.159)
Details:
HT: 300 kV
Cs: 0.01 mm (corrected by Cs corrector)
Pixel size: 1.13 A/pix
Dose per frame: 0.4629 electron/angstrom^2
Defocus Range: -0.8 μm to -2.5 μm.

NB: The pixel size in the TIFF header is not correct.
Files:
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Files:
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Rigden DJ, Fernández XM. (2023)
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Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)