EMPIAR-11262 - RedBeta177 oligomeric helical assembly bound to two complementary 27mer ssDNA oligonucleotides [4701 multi-frame micrographs composed of 50 frames each in MRC format]

EMPIAR-11262

RedBeta177 oligomeric helical assembly bound to two complementary 27mer ssDNA oligonucleotides [4701 multi-frame micrographs composed of 50 frames each in MRC format]

Publication:

Redβ₁₇₇ annealase structure reveals details of oligomerization and λ Red-mediated homologous DNA recombination

Newing TP, Brewster JL, Fitschen LJ, Bouwer JC, Johnston NP, Yu H, Tolun G

Nature communications 13 (2022)

PMID: 36163171

DOI: 10.1038/s41467-022-33090-6

Related EMDB entry:

EMD-26566

Deposited:

2022-08-02

Released:

2022-11-14

Last modified:

2022-11-14

Imageset size:

880.53 GB

Imageset DOI:

10.6019/EMPIAR-11262

Experimental metadata:

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Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Original size:   6,536,920,703 kilobytes
Compressed size:   923,308,551 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of helical RedB177 filaments in complex with complementary 27mer oligonucleotides

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

4701

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.84, 0.84)

Details:

The micrographs are unaligned micrograph movie files consisting of 50 raw movie frames in MRC format (accumulating 50 electrons per squared Angstrom over a 10 second exposure)

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Citations

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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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