EMPIAR-11262 - RedBeta177 oligomeric helical assembly bound to two complementary 27mer ssDNA oligonucleotides [4701 multi-frame micrographs composed of 50 frames each in MRC format]

EMPIAR-11262

RedBeta177 oligomeric helical assembly bound to two complementary 27mer ssDNA oligonucleotides [4701 multi-frame micrographs composed of 50 frames each in MRC format]

Publication:

Redβ₁₇₇ annealase structure reveals details of oligomerization and λ Red-mediated homologous DNA recombination

Newing TP, Brewster JL, Fitschen LJ, Bouwer JC, Johnston NP, Yu H, Tolun G

Nature communications 13 (2022)

PMID: 36163171

DOI: 10.1038/s41467-022-33090-6

Related EMDB entry:

EMD-26566

Deposited:

2022-08-02

Released:

2022-11-14

Last modified:

2022-11-14

Imageset size:

880.53 GB

Imageset DOI:

10.6019/EMPIAR-11262

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Original size:   6,536,920,703 kilobytes
Compressed size:   923,308,551 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of helical RedB177 filaments in complex with complementary 27mer oligonucleotides

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

4701

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.84, 0.84)

Details:

The micrographs are unaligned micrograph movie files consisting of 50 raw movie frames in MRC format (accumulating 50 electrons per squared Angstrom over a 10 second exposure)

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Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

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