EMPIAR-11198 - In situ cryo-electron tomography of E. amylovora cells infected by the jumbo bacteriophage RAY [multiple data sets in TIFF format]

EMPIAR-11198

In situ cryo-electron tomography of E. amylovora cells infected by the jumbo bacteriophage RAY [multiple data sets in TIFF format]

Publication:

Identifying the core genome of the nucleus-forming bacteriophage family and characterization of Erwinia phage RAY

Prichard A, Lee J, Laughlin TG, Lee A, Thomas KP, Sy AE, Spencer T, Asavavimol A, Cafferata A, Cameron M, Chiu N, Davydov D, Desai I, Diaz G, Guereca M, Hearst K, Huang L, Jacobs E, Johnson A, Kahn S, Koch R, Martinez A, Norquist M, Pau T, Prasad G, Saam K, Sandhu M, Sarabia AJ, Schumaker S, Sonin A, Uyeno A, Zhao A, Corbett KD, Pogliano K, Meyer J, Grose JH, Villa E, Dutton R, Pogliano J

Cell reports 42 (2023)

PMID: 37120812

DOI: 10.1016/j.celrep.2023.112432

Related EMDB entry:

EMD-27993 EMD-27973 EMD-28009 EMD-28006 EMD-28005 EMD-28007 EMD-28010 EMD-28008 EMD-28003 EMD-28004

Deposited:

2022-09-01

Released:

2023-10-03

Last modified:

2023-10-03

Imageset size:

244.34 GB

Imageset DOI:

10.6019/EMPIAR-11198

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Unaligned tilt-movie frames as non-normalized LZW-TIFF

Category:

tilt series

Image format:

TIFF

No. of images or tilt series:

950

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(4.265, 4.265)

Details:

Gain reference supplied in data/frames as MRC and requires a 270 CW rotation prior to application to TIFs. Fluence per tilt-movie is 2.19 electrons per square Angstrom. SerialEM metadata for 17 tilt-series is in data/frames/mdocs, Warp/M metadata in data/frames/

Files:

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Download a list of selected files.

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rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Unaligned tilt-movie frames as non-normalized LZW-TIFF

Category:

tilt series

Image format:

TIFF

No. of images or tilt series:

872

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(4.265, 4.265)

Details:

Gain reference supplied in data/frames as MRC and requires a 270 CW rotation prior to application to TIFs. Fluence per tilt-movie is 2.62 electrons per square Angstrom. SerialEM metadata for 15 tilt-series is in data/frames/mdocs, Warp/M metadata in data/frames/

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

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