EMPIAR-11070 - Tilt series of SARS-CoV-2 virions, Wuhan/Alpha/Beta/Delta variants [multiple data sets in MRC format]

EMPIAR-11070

Tilt series of SARS-CoV-2 virions, Wuhan/Alpha/Beta/Delta variants [multiple data sets in MRC format]

Publication:

Electron cryotomography of SARS-CoV-2 virions reveals cylinder-shaped particles with a double layer RNP assembly

Calder LJ, Calcraft T, Hussain S, Harvey R, Rosenthal PB

Communications Biology 5 (2022)

DOI: 10.1038/s42003-022-04183-1

Related EMDB entry:

EMD-15183 EMD-15185 EMD-15181 EMD-15182

Deposited:

2022-06-15

Released:

2022-11-11

Last modified:

2022-11-11

Imageset size:

129.42 GB

Imageset DOI:

10.6019/EMPIAR-11070

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
tilt series

Image Sets

1. Unaligned tilt movies of SARS-CoV-2 virions (Wuhan strain)

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

533

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(2.22, 2.22)

Details:

Movies have 4 frames each, corresponding to 2.38e-/A^2 per tilt. Tilt series collected +/-60deg in dose symmetric sequence, grouping 2 (0,+3,-3,-6,+6,+9,etc...). Tilt axis rotation angle is 177deg.

Files:

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2. Tilt series of SARS-CoV-2 virions (Alpha variant B.1.1.7)

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(2.58, 2.58)

Details:

2.5e-/A^2 dose per tilt. Tilt series collected +/-57deg in dose symmetric sequence, grouping 2 (0,+3,-3,-6,+6,+9,etc...). Tilt axis rotation angle is -2deg.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Tilt series of SARS-CoV-2 virions (Beta variant B.1.351)

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(2.58, 2.58)

Details:

2.5e-/A^2 dose per tilt. Tilt series collected +/-57deg in dose symmetric sequence, grouping 2 (0,+3,-3,-6,+6,+9,etc...). Tilt axis rotation angle is -2deg.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Tilt series of SARS-CoV-2 virions (Delta variant B.1.617)

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(2.58, 2.58)

Details:

2.0e-/A^2 dose per tilt. Tilt series collected +/-60deg in dose symmetric sequence, grouping 2 (0,+3,-3,-6,+6,+9,etc...). Tilt axis rotation angle is -2deg.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

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