EMPIAR-11069 - Cryo electron microscopy micrographs of high molecular weight fractions from yeast native cell extracts [multiple data sets in MRC format]

EMPIAR-11069

Cryo electron microscopy micrographs of high molecular weight fractions from yeast native cell extracts [multiple data sets in MRC format]

Publication:

Delineating organizational principles of the endogenous L-A virus by cryo-EM and computational analysis of native cell extracts

Schmidt L, Tüting C, Kyrilis FL, Hamdi F, Semchonok DA, Hause G, Meister A, Ihling C, Stubbs MT, Sinz A, Kastritis PL

Communications biology 7 (2024)

PMID: 38730276

DOI: 10.1038/s42003-024-06204-7

Related EMDB entry:

EMD-15214 EMD-15189 EMD-15215

Deposited:

2022-06-16

Released:

2024-01-23

Last modified:

2024-01-23

Imageset size:

4.66 TB

Imageset DOI:

10.6019/EMPIAR-11069

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (FoilHole*.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   9,032,250,836 kilobytes
Compressed size: 5,003,097,717 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Multi-frame micrographs for Saccharomyces cerevisiae native cell extract fraction

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

2728

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(3.177, 3.177)

Details:

Cryo-EM micrographs of Saccharomyces cerevisiae fractionated cell extract. Analyzed fraction is enriched in the endogenous L-A helper virus and Ribo- and Polysomes.
Micrographs were acquired at a dose of 30 Å/px and a pixel size of 3.177.

Files:

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2. Multi-frame micrographs for Saccharomyces cerevisiae native cell extract fraction

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

7813

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(3.177, 3.177)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Multi-frame micrographs for Saccharomyces cerevisiae native cell extract fraction

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

2254

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(3.177, 3.177)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Multi-frame micrographs for Saccharomyces cerevisiae native cell extract fraction

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

4033

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.5678, 1.5678)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

5. Multi-frame micrographs for Saccharomyces cerevisiae native cell extract fraction

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

2978

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.5678, 1.5678)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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